• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse VEGF-B in direct ELISAs and Western blots. Recognizes both the VEGF-B    ₁₆₇ and VEGF-B    ₁₈₆ isoforms. In Western blots, no cross-reactivity with recombinant human (rh) VEGF, rhVEGF-C, rhVEGF-D, rhCTGF, rhPlGF, rhLDGF, or rhPDGF is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 58013

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human VEGF-B    ₁₆₇    
  Pro22-Arg188    
  Accession # AAB06274

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human VEGF-B    ₁₆₇ (Catalog # )    
  Recombinant Mouse VEGF-B    ₁₆₇ (Catalog # )    
  Recombinant Mouse VEGF-B    ₁₈₆ (Catalog # )

• 
  

• Immunohistochemistry

  3-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-B

Vascular endothelial growth factor B (VEGF-B), also known as vascular endothelial growth factor-related factor (VRF), is a member of the VEGF family of growth factors that share structural and functional similarity (1, 2). Five mammalian members, including VEGF-A, -B, -C, -D and PlGF, have been identified. VEGF family members are disulfide-linked dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis and lymphangiogenesis. VEGF-B is expressed in most tissues, especially in heart, skeletal muscle and pancreas. In many tissues, VEGF-B is co-expressed and can heterodimerize with VEGF (3). By alternative splicing, two isoforms of mature VEGF-B containing 167 or 186 amino acid (aa) exist (3, 4). The two VEGF-B isoforms have identical amino-terminal cysteine-knot VEGF homology domains but the carboxyl end of VEGF-B167 differs from that of VEGF-B186 by the presence of a highly basic cysteine-rich heparin binding domain. Whereas VEGF-B186 is a secreted diffusible protein, VEGF-B167 is sequestered into the cell matrix after secretion. Both VEGF-B isoforms bind VEGF receptor 1 (VEGF R1), but not VEGF R2 or VEGF R3 (5). On endothelial cells, ligation of VEGF R1 by VEGF-B has been shown to regulate the expression and activity of urokinase type plasminogen activator and plasminogen activator inhibitor 1. VEGF-B167 and a proteolytically processed form of VEGF-B186 (VEGF-B127) also bind neuropilin-1 (NP-1), a type I transmembrane receptor for semaphorins/collapsins, ligands involved in neuron guidance (6). Besides VEGF-B, NP-1 has been shown to bind PLGF-2, VEGF165 and VEGF R1 (6, 7). The many interactions of NP-1 with VEGF ligands and receptor suggests that NP-1 may function as a regulator of angiogenesis (7).

• References:

1. Li, X. and U. Eriksson (2001) Int. J. Biochem Cell Biol. 33:421.

2. Olofsson, B. et al. (1999) Curr. Opin. Biotechnol. 10:528.

3. Olofsson, B. et al. (1996) Proc. Nat. Acad. Sci. USA 93:2576.

4. Grimmond, S. et al. (1996) Benome Res. 6:124.

5. Olofsson, B. et al. (1998) Proc. Nat. Acad. Sci. USA 95:11709.

6. Makinen, T. et al. (1999) J. Biol. Chem. 274:21217.

7. Fuh, G. et al. (2000) J. Biol. Chem. 275:26690.

• Long Name:

  Vascular Endothelial Growth Factor B

• Entrez Gene IDs:

  7423 (Human); 22339 (Mouse)

• Alternate Names:

  vascular endothelial growth factor B; VEGFB; VEGF-B; VEGF-related factor; VRFVEGFL