• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse and rat CBP.

• Source

  Monoclonal Mouse IgG    _2B Clone # 257003

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human CBP    
  Asn2141-Leu2442    
  Accession # Q92793

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.4 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  Immersion fixed HeLa human cervical epithelial carcinoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CBP

The CBP (CREB-binding protein) is a histone acetyltransferase and transcriptional coactivator that regulates RNA polymerase II transcription. In addition to histone acetylation, CBP also acetylates various transcription factors, including the tumor suppressor protein p53, to enhance sequence-specific DNA binding.

• Long Name:

  CREB-binding Protein

• Entrez Gene IDs:

  1387 (Human); 12914 (Mouse); 54244 (Rat)

• Alternate Names:

  CBP; CBPRTS; CREB binding protein; CREB-binding protein; CREBBP; EC 2.3.1.48; KAT3A; RSTS; RTS; Rubinstein-Taybi syndrome