• Species Reactivity

  Human

• Specificity

  Detects human CD133. Stains human CD133 transfectants but not irrelevant transfectants in flow cytometry.

• Source

  Monoclonal Mouse IgG    _2A Clone # 170411

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  NS0 mouse myeloma cell line transfected with human CD133    
  Accession # O43490

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 700

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  5 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types.  CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells.   

• Entrez Gene IDs:

  8842 (Human)

• Alternate Names:

  AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1