• Species Reactivity

  Sheep

• Specificity

  Detects sheep IgG heavy and light chains in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with mouse IgG, rabbit IgG, human IgG, and chicken IgY is observed.

• Source

  Polyclonal Donkey IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Sheep IgG

• Formulation

  Sodium Phosphate and Proclin 300.

• Label

  HRP (Horseradish Peroxidase)

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  Use at a 1:1000 dilution.

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• Simple Western

  Use at a 1:50 dilution.

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Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Do not freeze.    

• 6 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

• Long Name:

  Immunoglobulin G

• Alternate Names:

  IgG; immunoglobulin heavy constant gamma 1 (G1m marker)