• Species Reactivity

  Human

• Specificity

  Detects human IL‑1 alpha /IL‑1F1 in ELISAs and western blots.    
  In ELISAs, this antibody does not cross-react with recombinant human (rh) IL-1 beta, -2, -3, -4, -5, -6, -7, -8, -9, -10, -11, -12, -13, rmIL-1 alpha, -1 beta, -3, -4, -5, -6, -7, -9, or -13.

• Source

  Recombinant Monoclonal Mouse IgG    _2A Clone # 4414R

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  E. coli-derived recombinant human IL‑1 alpha /IL‑1F1    
  Ser113-Ala271    
  Accession # P01583

• Formulation

  Supplied as a solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

• Human IL-1 alpha /IL-1F1 Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  2-8 µg/mL 

  Human IL‑1 alpha /IL‑1F1 Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Human IL‑1 alpha /IL‑1F1 Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Human IL-1 alpha/IL-1F1 Protein (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize IL‑1 alpha /IL‑1F1-induced proliferation in the D10.G4.1 mouse helper T cell line. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The Neutralization Dose (ND     ₅₀) is typically 0.05-0.15 µg/mL in the presence of 50 pg/mL Recombinant Human IL‑1 alpha /IL‑1F1.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C, as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-1 alpha/IL-1F1

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts, and glia (oligodendroglia, astrocytes, and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

• Long Name:

  Interleukin 1 alpha

• Entrez Gene IDs:

  3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)

• Alternate Names:

  Hematopoietin-1; IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha