• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects endogenous human, mouse, and rat TRAF-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human TRAF-1, -2, -4, -5, or -6.

• Source

  Monoclonal Rat IgG    _2A Clone # 433113

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TRAF-3    
  Met1-Pro543    
  Accession # NP_663778

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRAF-3

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Seven members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. Two isoforms of TRAF-3 have been identified, a 568 amino acid, 64 kDa protein (isoform 1, also known as CD40BP and CRAF1) and a 543 amino acid, 62 kDa protein (isoform 2, also known as CAP1 and LAP1). TRAF-3 has been shown bind specifically to the PEQET sequence motif on the cytoplasmic domain of CD40. TRAF-3 and TRAF-6 have also been identified as being involved in TLR signaling events.

• Long Name:

  TNF Receptor-Associated Factor 3

• Entrez Gene IDs:

  7187 (Human); 22031 (Mouse)

• Alternate Names:

  CAP1; CAP-1CD40 receptor associated factor 1; CD40 associated protein 1; CD40 binding protein; CD40 receptor-associated factor 1; CD40-binding protein; CD40bp; CRAF1; CRAF1CAP1; LAP1; LAP1CD40BP; LMP1-associated protein 1; TNF receptor-associated factor 3; TRAF3; TRAF-3