• Species Reactivity

  Mouse

• Specificity

  Detects mouse Serpin A6 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Serpin A1, A5, B8, C1, D1, E1, E2, F1, F2, I1, recombinant human Serpin A3, A4, B6, B9, G1, I2, or recombinant rat Serpin A6 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 433903

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Serpin A6    
  Val23-Ala397    
  Accession # Q06770

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Serpin A6

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A6

Serpin A6 (also known as corticosteroid-binding globulin/CBG and Transcortin) is a 50‑60 kDa, secreted glycoprotein member of the serpin family of protease inhibitors. It is produced by hepatocytes and transports > 90% of circulating cortisol. Although Serpin A6 is a serpin family member, it shows no protease inhibition. Mature mouse Serpin A6 is 375 amino acids (aa) in length. Over aa 23‑397, mouse Serpin A6 shares 78% and 57% aa sequence identity with rat and human Serpin A6, respectively.

• Entrez Gene IDs:

  866 (Human); 12401 (Mouse); 299270 (Rat)

• Alternate Names:

  antitrypsin), member 6; CBG; CBGcorticosteroid-binding globulin; corticosteroid binding globulin; member 6; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); transcortin