• Species Reactivity

  Human

• Specificity

  Detects human IGDCC3 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 920038

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human embryonic kidney cell line HEK293-derived recombinant human IGDCC3    
  Met1-Gly640    
  Accession # Q81VU1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 µg/mL

  See below

• 
  

• Flow Cytometry

  0.25 µg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGDCC3

IGDCC3 (Immunoglobulin superfamily DCC subclass member 3), also known as Punc, is 814 aa single-pass type membrane protein and shares 84% aa identity with mouse IGDCC3.  It is a member of the neural cell adhesion molecule family and contains two fibronectin type III repeats and four immunoglobulin-like domains.  IDGCC3 is high expressed in the brain and has been suggested to play a role in motor coordination.  It has also been suggested to play a role in breast cancer. 

• Long Name:

  Immunoglobulin Superfamily, DCC Subclass, Member 3

• Entrez Gene IDs:

  9543 (Human); 19289 (Mouse)

• Alternate Names:

  HsT18880; IGDCC3; immunoglobulin superfamily DCC subclass member 3; immunoglobulin superfamily, DCC subclass, member 3; PUNC; Putative neuronal cell adhesion moleculePUNCHsT18880