• Species Reactivity

  Human

• Specificity

  Detects human Carnosine Dipeptidase 1/CNDP1 in direct ELISAs.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 352213

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Carnosine Dipeptidase 1/CNDP1    
  Pro28-His507    
  Accession # Q96KN2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human Carnosine Dipeptidase 1/CNDP1 (Catalog # ),

• 
  

• Neutralization

  Measured by its ability to neutralize Recombinant Human Carnosine Dipeptidase 1/CNDP1 (0.5 µg/mL, Catalog # ) cleavage of the fluorogenic peptide substrate L-Carnosine (0.5 mM). The Neutralization Dose (ND    ₅₀) is typically 0.6 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carnosine Dipeptidase 1/CNDP1

The human CNDP1 gene encodes carnosine dipeptidase 1, a member of the M20 family of metalloproteases (1, 2). Also known as X-His dipeptidase, glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1), CNDP1 is a secreted dipeptidase with a narrow specificity for Xaa-His dipeptides including those with Xaa = beta -Ala (carnosine) and Xaa = gamma -aminobutyric acid (homocarnosine), two naturally occurring dipeptides with potential neuroprotective and neurotransmitter fucntions in the brain. In comparison, a closely related protein known as CNDP2, CPGL or CN2, is a cytosolic nonspecific dipeptidase. CNDP1 consists of a signal peptide (residues 1 to 26) and the mature chain (residues 27 to 507). The amino acid sequence of human CNDP1 is 97%, 85%, 82% and 67% identical to that of chimpanzee, canine, mouse/rat and chicken.

• References:

1. Teufel, M. et al. (2004) J. Biol. Chem. 278:6521.

2. Bauer, K. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1022, Academic Press, San Diego.

• Entrez Gene IDs:

  84735 (Human)

• Alternate Names:

  beta-Ala-His dipeptidase; Carnosinase 1; carnosine dipeptidase 1 (metallopeptidase M20 family); Carnosine Dipeptidase 1; CN1MGC102737; CNDP dipeptidase 1; CNDP1; CPGL2; CPGL2MGC142072; EC 3.4.13.20; Glutamate carboxypeptidase-like protein 2; HsT2308; MGC10825; Serum carnosinase