• Species Reactivity

  Human

• Specificity

  Detects human NTH1 in Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 208521

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human NTH1    
  Met1-Leu312    
  Accession # P78549

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NTH1

NTH1 is a DNA glycosylase with apurinic lyase activity. It functions in the repair of oxidative damage to DNA through the BER (base excision repair) pathway.

• Long Name:

  Nth Endonuclease III-like 1

• Entrez Gene IDs:

  4913 (Human)

• Alternate Names:

  EC 4.2.99.18; endonuclease III-like protein 1; nth endonuclease III-like 1 (E. coli); NTH1; NTHL1; OCTS3; OCTS3NTH1nth (E.coli endonuclease III)-like 1