• Species Reactivity

  Mouse

• Specificity

  Detects mouse Serpin F2/ alpha     ₂‑Antiplasmin in Western blots and direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Serpin F2 and no cross-reactivity with rhSerpin A1, A3, A4 or recombinant mouse Serpin A5, C1, or D1 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 209126

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Serpin F2/ alpha     ₂‑Antiplasmin    
  Val28-Lys491    
  Accession # Q61247

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Serpin F2/ alpha     ₂‑Antiplasmin (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin F2/alpha 2-Antiplasmin

Serpin F2 is a member of the Serpin superfamily and the primary physiological inhibitor of the serine protease plasmin, which is responsible for the dissolution of fibrin clots (1, 2). In addition to plasmin, Serpin F2 is also an efficient inhibitor of trypsin and chymotrypsin (3). Liver and kidney are major sites of Serpin F2 production and other tissues such as muscle, intestine, central nervous system, and placenta also express its mRNA at a moderate level (3). The tissue expression pattern of Serpin F2 indicates that it is a key regulator of plasmin-mediated proteolysis in these tissues. Mouse Serpin F2 is synthesized as a 491 amino acid precursor with a 27 amino acid signal peptide. The secreted protein has a short propeptide (residues 28 to 39) and a mature chain (residues 40 to 491). For human Serpin F2, the presence of the propeptide did not affect its ability to inhibit plasmin but reduced its cross-linking ability to fibrin (4).

• References:

1. Menoud, P.-A. et al. (1996) J. Clin. Invest. 97:2478.

2. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.

3. Potempa, J. et al. (1988) Science. 241:699.

4. Sumi, Y. et al. (1989) J. Biochem. 106:703.

• Entrez Gene IDs:

  5345 (Human); 18816 (Mouse)

• Alternate Names:

  AAPclade F (alpha-2 antiplasmin; alpha 2-Antiplasmin; Alpha-2-AP; Alpha-2-PI; Alpha-2-plasmin inhibitor; pigment epithelium derived factor), member 2; Serpin F2; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 2