• Species Reactivity

  Human

• Specificity

  Detects human 17 beta ‑HSD1/HSD17B1 in ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 860020

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human 17 beta ‑HSD1/HSD17B1    
  Ala2-Gln328    
  Accession # P14061

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.25 µg/mL

  See below

• 
  

• Simple Western

  2.5 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 17 beta-HSD1/HSD17B1

HSD17B1 (Hydroxysteroid 17-beta dehydrogenase type 1; also 17  beta ‑HSD1 and E2DH) is a 33-34 kDa member of the short chain dehydrogenase/reductase (SDR) superfamily of molecules. It is a cytoplasmic breast, placental and gonadal enzyme that both converts DHEA to estrogen A-diol, and inactivates DHT through oxidation and reduction. Human HSD17B1 is 328 amino acids (aa) in length. It contains an NADP binding site (aa 10-38) and a steroid catalytic site (aa 210-221). HSD17B1 functions as a 68 kDa noncovalently-linked homodimer. Over aa 2-328, human HSD17B1 shares 65% aa identity with mouse HSD17B1.

• Long Name:

  17 beta Hydroxysteroid Dehydrogenase 1

• Entrez Gene IDs:

  3292 (Human); 15485 (Mouse); 25322 (Rat)

• Alternate Names:

  17 betaHSD1; 17 beta-HSD1; 17-beta-HSD 1; 17-beta-hydroxysteroid dehydrogenase type 1; 20 alpha-hydroxysteroid dehydrogenase; 20-alpha-HSD; E17KSR; E2DH; EC 1.1.1.62; EDH17B1; EDH17B2; EDH17B2EDHB17; EDHB17; estradiol 17-beta-dehydrogenase 1; estradiol 17-beta-dehydrogenase-1; HSD17; HSD17B1; hydroxysteroid (17-beta) dehydrogenase 1 isoform; hydroxysteroid (17-beta) dehydrogenase 1; MGC138140; Placental 17-beta-hydroxysteroid dehydrogenase; SDR28C1; short chain dehydrogenase/reductase family 28CE, member 1