• Species Reactivity

  Human

• Specificity

  Detects human Porimin in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 297617

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Porimin    
  His27-Ser149    
  Accession # AAG21694

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Porimin

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• Flow Cytometry

  2.5 µg/10    ⁶ cells

  Jurkat human acute T cell leukemia cell line

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• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Porimin

Porimin is a widely expressed mucin-related transmembrane glycoprotein. Cross-linking of Porimin leads to membrane disruption and cell death.

• Long Name:

  Pro-Oncosis Receptor Inducing Membrane Injury

• Entrez Gene IDs:

  114908 (Human)

• Alternate Names:

  KCT3; KCT-3; KCT3keratinocytes associated transmembrane protein 3; Keratinocytes-associated transmembrane protein 3; Porimin; PORIMINporimin; pro oncosis receptor inducing membrane injury; Pro-oncosis receptor inducing membrane injury; serine/threonine-rich receptor; TMEM123; transmembrane protein 123PORMIN