• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse Glypican 5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant mouse Glypican 5 is observed and no cross‑reactivity with recombinant human Glypican 2, 3 or 6 is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 297716

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Glypican 5    
  Glu25-Thr554    
  Accession # P78333

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Glypican 5 (Catalog # )    
  Recombinant Mouse Glypican 5 (Catalog # )

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• Flow Cytometry

  2.5 µg/10    ⁶ cells

  H9 human cutaneous T lymphoma cell line

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glypican 5

Glypican 5 (GPC5) belongs to the Glypican family of heparan sulfate proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. Glypican 5 is expressed primarily in embryonic neurons and mesenchyme.

• Entrez Gene IDs:

  2262 (Human); 103978 (Mouse)

• Alternate Names:

  bA93M14.1; Glypican 5; glypican proteoglycan 5; glypican-5; GPC5