详细说明
- Species ReactivityHuman
- SpecificityDetects human Endothelin‑1 C-Terminus. Recognizes the C-terminal region of mature Endothelin-1, -2, and -3 (amino acid residues 15‑21).
- SourceMonoclonal Rat IgG 2B Clone # 3G10
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenHuman Endothelin-1
- FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- ELISAThis clone has been shown to effectively function as an ELISA capture antibody.
- Immunocytochemistry8-25 µg/mLSee below
Immunocytochemistry | Endothelin‑1 in HUVEC Human Cells. Endothelin‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Rat Anti-Human Endothelin‑1 C‑Terminus Monoclonal Antibody (Catalog # MAB3440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # ) and counterstained with DAPI (blue). View our protocol for . |
- ReconstitutionReconstitute at 0.5 mg/mL in sterile PBS.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15‑21).
- Entrez Gene IDs:1906 (Human); 13614 (Mouse); 24323 (Rat)
- Alternate Names:EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1