• Species Reactivity

  Mouse

• Specificity

  Detects mouse Desert Hedgehog/Dhh C-Terminus in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross‑reactivity with recombinant mouse (rm) Shh C‑terminus peptide (aa 199‑437) and rmIhh C-terminus peptide (aa 241‑449) is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse Desert Hedgehog/Dhh C-Terminus    
  Cys199-Gly396    
  Accession # Q61488

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Desert Hedgehog (C23II), N-Terminus (Catalog # )

• 
  

• Immunohistochemistry

  5-15 µg/mL

  Perfusion fixed frozen sections of mouse spinal cord

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desert Hedgehog/Dhh

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa N-terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling (1‑4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh) (6, 7). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (8, 9). It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation (10, 11). It also promotes the deposition of basal lamina surrounding seminiferous tubules (8). In humans, mutations of Dhh are associated with pure gonadal dysgenesis (12). Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum (13). Its up‑regulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis (14). Dhh is also expressed by Schwann cells and is up‑regulated following nerve injury (15, 16). It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (15).

• References:

1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343.

2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369.

3. Porter, J.A. et al. (1995) Nature 374:363.

4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630.

5. Pathi, S. et al. (2001) Mech. Dev. 106:107.

6. Echelard, Y. et al. (1993) Cell 75:1417.

7. Chang, D.T. et al. (1994) Development 120:3339.

8. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392.

9. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298.

10. Yao, H.H.-C. et al. (2002) Genes Dev. 16:1433.

11. Park, S.Y. et al. (2007) Endocrinology 148:3704.

12. Canto, P. et al. (2004) J. Clin. Endocrinol. 89:4480.

13. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.

14. Chen, X. et al. (2007) Cancer Sci. 98:68.

15. Parmantier, E. et al. (1999) Neuron 23:713.

16. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.

• Entrez Gene IDs:

  50846 (Human); 13363 (Mouse)

• Alternate Names:

  desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; Desert Hedgehog; DHH; HHG-3MGC35145