• Species Reactivity

  Mouse

• Specificity

  Detects mouse Aminopeptidase P2/XPNPEP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human Aminopeptidase P2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase P2/XPNPEP2    
  Tyr22-Ala650    
  Accession # Q91Y31

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Aminopeptidase P2/XPNPEP2 (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse Aminopeptidase P2/XPNPEP2 (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase P2/XPNPEP2

The mouse XPNPEP2 gene encodes Aminopeptidase P2 (APP2), which is also known as X-prolyl Aminopeptidase 2 or membrane bound Aminopeptidase P (1, 2). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, Aminopeptidase P1, Aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (3). Mammalian APP2 are predicted to be GPI-anchored membrane proteases and their biological functions have been reviewed (4). Mouse APP2 is expressed in many adult tissues and in embryos (5). The purified rmAPP2 corresponds to the ectodomain and is shown here to be an active aminopeptidase, removing an N-terminal amino acid from a peptide that contains a Pro residue at the second position.

• References:

1. Kawai, J. et al. (2001) Nature 409:685.

2. Shibata, K. et al. (2000) Genome Res. 10:1757.

3. Barrett, A.J. et al. (2004) Handbook of Proteolytic Enzymes, Elsevier Academic Press, San Diego.

4. Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego.

5. Ersahin C. et al. (2003) Arch. Biochem. Biophys. 417:131.

• Entrez Gene IDs:

  7512 (Human); 170745 (Mouse)

• Alternate Names:

  Aminoacylproline aminopeptidase; Aminopeptidase P2; APP2; EC 3.4.11.9; mAmP; Membrane-bound aminopeptidase P; Membrane-bound AmP; Membrane-bound APP; xaa-Pro aminopeptidase 2; XPNPEP2; X-Pro aminopeptidase 2; X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound