• Species Reactivity

  Mouse

• Specificity

  Detects mouse Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A5, recombinant mouse (rm) Serpin C1, and rmSerpin F2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Serpin D1/Heparin Cofactor II    
  Glu24-Ser478    
  Accession # P49182

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Serpin D1/Heparin Cofactor II (Catalog # )

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse Serpin D1/    
  Heparin Cofactor II (Catalog # ),

• 
  

• Neutralization

  Measured by its ability to neutralize Recombinant Mouse Serpin D1 (0.475 µg/mL, Catalog # ) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # ) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ). The Neutralization Dose (ND    ₅₀) is typically 1.23 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin D1/Heparin Cofactor II

Serpin D1 is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). Mouse Serpin D1 is synthesized as a 478 amino acid precursor with a 23 amino acid signal peptide (4). The mature chain (residues 24 to 478) is secreted.

• References:

1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.

2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.

3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.

4. Zhang, G.S. et al. (1994) Biochemistry 33:3632.

• Entrez Gene IDs:

  3053 (Human); 15160 (Mouse)

• Alternate Names:

  HC2; HC2LS2; HCF2; HCF2clade D (heparin cofactor), member 1; HC-II; Heparin Cofactor II; HLS2HCII; leuserpin 2; LS2; Protease inhibitor leuserpin-2; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1