• Species Reactivity

  Mouse

• Specificity

  Detects mouse Indian Hedgehog (Ihh) N-terminal peptide in Western blots. In Western blots, approximately 80% cross-reactivity with recombinant human Shh N-terminal peptide and recombinant mouse (rm) Shh N-terminal peptide is observed, and less than 1% cross‑reactivity with rmIhh C-terminal peptide (amino acids 241-449) is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse Indian Hedgehog    
  Cys28-Gly202    
  Accession # P97812

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse Indian Hedgehog (C28II), N-Terminus

• 
  

• Immunohistochemistry

  5-15 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Indian Hedgehog/Ihh

The hedgehog (hh) gene encoding a secreted protein was originally identified in Drosophila as a segment polarity gene. The vertebrate homologues of Hh comprise several proteins including sonic hedgehog (Shh), Indian hedgehog (Ihh), and Desert hedgehog (Dhh) (1). Hedgehog proteins are important signaling molecules during embryonic development and are highly conserved within and across species (1). Mouse and human Ihh share 100% amino acid (aa) sequence identity in the signaling domain, while mouse Ihh and Shh share 90% aa identity in the N-terminal signaling domain. Ihh mRNA expression is detected in fetal lung, gut, stomach, liver, kidney, pancreas, and strongly in cartilage, in growth regions of the developing bone (2, 3). Ihh, along with parathyroid hormone related protein, regulate the rate of chondrocyte proliferation and differentiation (4). Ihh is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells (5).

Mouse Ihh cDNA encodes a 411 aa polypeptide with a predicted 27 aa signal peptide. This polypeptide is cleaved to generate a 45 kDa precursor protein that undergoes the same post-translation processing as Shh (3). An autocatalytic reaction yields a 19 kDa amino-terminal domain Ihh-N protein that retains all known signaling capabilities, and a 23 kDa carboxy-terminal domain Ihh-C protein (3). Since hydrophobic modifications to Shh, including the substitution of the N-terminal cysteine residue with two hydrophobic isoleucine residues, can also increase its potency (6), a similar modification was made for Ihh. This modified form also shows increased potency in a bioassay measuring induction of alkaline phosphatase. At the cell surface, Hedgehog activity is mediated by a multicomponent receptor complex involving the 12-pass transmembrane protein Patched (Ptc) which binds Hedgehogs with high affinity and Smoothened (Smo), a signaling seven transmembrane G-protein coupled receptor (1).

• References:

1. Ingham, P. and A. McMahon (2001) Genes & Dev. 15:3059.

2. Marigo, V. et al. (1995) Genomics 28:44.

3. Valentini, R.P. et al. (1997) J Biol Chem. 272:8466.

4. Vortkamp, A. et al. (1996) Science 273:613.

5. Byrd, N. et al. (2002) Development 129:361.

6. Taylor, F.R. et al. (2001) Biochemistry 40:4359.

• Entrez Gene IDs:

  3549 (Human); 16147 (Mouse)

• Alternate Names:

  BDA1; BDA1Indian hedgehog homolog (Drosophila); HHG2; HHG-2; Ihh; Indian hedgehog (Drosophila) homolog; Indian hedgehog homolog; indian hedgehog protein; Indian Hedgehog