• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL‑17B in ELISAs and Western blots. In sandwich immunoassays, approximately 40% cross-reactivity with recombinant human IL-17B is observed and less than 0.3% cross-reactivity with recombinant mouse (rm) IL‑17, rmIL-17C, rmIL-17D, rmIL-17E, and    
  rmIL‑17F is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse IL-17B    
  His21-Phe180    
  Accession # Q9QXT6

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  Recombinant Mouse IL‑17B (Catalog # )

• 
  

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 0.4 - 2 µg/mL of this antibody will block 50% of the binding of 10 μg/mL of biotinylated Recombinant Mouse IL-17 beta R Fc Chimera to immobilized Recombinant Mouse IL-17B (Catalog # ) coated at 2 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  2.5 µg/10    ⁶ cells

  See below

• 
  

• Mouse IL-17B Sandwich Immunoassay

  Reagent    

• ELISA Capture (Matched Antibody Pair)

  0.2-0.8 µg/mL 

  Mouse IL‑17B Antibody (Catalog # )

• ELISA Detection (Matched Antibody Pair)

  0.1-0.4 µg/mL 

  Mouse IL‑17B Biotinylated Antibody (Catalog # )

• ELISA Standard

   

  Recombinant Mouse IL-17B Protein (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17B

The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cystine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL-17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Two receptors (IL-17 R and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional orphan type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and
IL‑17 RE, have also been reported (1‑4). Mouse IL-17B cDNA encodes a 180 amino acid residue (aa) protein with a putative 20 aa signal peptide (5). Mouse and human IL-17B share 88% aa sequence identity. Among IL-17 family members, mouse IL-17B is most closely related to mouse IL-17D, sharing 33% aa sequence homology. IL-17B is expressed highly in spinal cord, and at lower levels in brain, kidney, lung, small intestine, prostate, testes, pancreas, adrenal gland and trachea (5‑7). Expression of IL-17B has also been detected in chondrocytes in articular cartilage (2). IL-17B binds the IL-17B receptor but not IL-17 R and exhibits bioactivities distinct from those of IL-17 (5, 6).

• References:

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.

2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.

3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.

4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.

5. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.

6. Li, H. et al. (2000) Proc. Natl. Acad. Sci. USA 97:773.

• Long Name:

  Interleukin 17B

• Entrez Gene IDs:

  27190 (Human); 56069 (Mouse)

• Alternate Names:

  Cytokine Zcyto7; cytokine-like protein ZCYTO7; IL17B; IL-17B; IL-17Binterleukin-17 beta; IL20; IL-20interleukin-17B; interleukin 17B; interleukin 20; Interleukin-20; MGC138900; MGC138901; neuronal interleukin-17 related factor; Neuronal interleukin-17-related factor; NIRF; ZCYTO7interleukin-20