• Species Reactivity

  Mouse

• Specificity

  Detects recombinant mouse Shh C-terminal peptide in direct ELISAs and Western blots. Does not cross-react in direct ELISAs with rmDhh C- or N-terminal peptides, rmIhh C- or N-terminal peptides, rhShh N-terminal peptide, or rmShh N-terminal peptide.

• Source

  Monoclonal Rat IgG    _2A Clone # 55626

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse Sonic Hedgehog    
  Cys199-Ser437    
  Accession # Q62226.1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Sonic Hedgehog C-Terminus

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sonic Hedgehog/Shh

Sonic Hedgehog (Shh) is a hedgehog protein that is instrumental in patterning the early embryo. It is cleaved by autoproteolysis to release an N-terminal signaling peptide that binds the multicomponent receptor complex containing the multi-pass transmembrane proteins Patched and Smoothened. The C-terminal peptide carries the protease domain which acts only in CIS. It also acts on a cholesterol transferase that covalently transfers the cholesterol molecule to the C-terminus of Shh-N.

• Entrez Gene IDs:

  6469 (Human); 20423 (Mouse)

• Alternate Names:

  HHG1; HHG-1; HLP3; HPE3; MCOPCB5sonic hedgehog (Drosophila) homolog; Shh; SMMCIsonic hedgehog homolog (Drosophila); sonic hedgehog homolog; sonic hedgehog protein; Sonic Hedgehog; TPT; TPTPS