详细说明
- Species ReactivityHuman
- SpecificityDetects human ERMAP in direct ELISAS.
- SourceMonoclonal Mouse IgG 2B Clone # 767504
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenMouse myeloma cell line NS0-derived recombinant human ERMAP
His30-Ala155
Accession # Q96PL5 - FormulationLyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
- LabelUnconjugated
- Recommended
ConcentrationSample
- Western Blot2 µg/mLSee below
| Western Blot | Detection of Human ERMAP by Western Blot. Western blot shows lysates of human erythrocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ERMAP Monoclonal Antibody (Catalog # MAB4928) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for ERMAP at approximately 63 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
- ReconstitutionSterile PBS to a final concentration of 0.5 mg/mL.
- ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
- Stability & StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
ERMAP (erythrocyte membrane-associated protein; also Scianna blood group antigen) is a 60‑66 kDa member of the BTN/MOG family, Ig-Superfamily of proteins. It is expressed on erythrocytes and erythrocyte precursors and likely serves as a cell adhesion molecule. Mature human ERMAP is a 446 amino acid (aa) type I transmembrane glycoprotein. It possesses a 126 aa extracellular domain (ECD) (aa 30‑155) that contains one V-type Ig-like domain (aa 30‑140). Single aa changes at Gly35, Glu47, Gly57, Pro60 and Arg81 generate distinct antigens of the Scianna blood group. There is one alternate start site at Met91 and a splice variant that shows an 11 aa substitution for aa 103‑475. Over aa 30‑155, human ERMAP is 56% aa identical to mouse ERMAP. In contrast to human ERMAP ECD, mouse ERMAP ECD contains one extra Ig-like domain.
- Long Name:Erythroblast Membrane-associated Protein
- Entrez Gene IDs:114625 (Human); 27028 (Mouse); 298485 (Rat)
- Alternate Names:ERMAP; erythroblast membrane-associated protein (RD and SC blood groups); erythroblast membrane-associated protein (Scianna blood group); erythroblast membrane-associated protein; erythroid membrane-associated protein; hERMAP; MGC118810; MGC118811; PRO2801; Radin blood group (Rd); Radin blood group antigen; Radin blood group; RD; RDMGC118812; SC; Scianna blood group (Sc); Scianna blood group antigen; Scianna blood group; SCMGC118813
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