• Species Reactivity

  Mouse

• Specificity

  Detects mouse Prostasin/Prss8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Prostasin is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 430814

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Prostasin/Prss8    
  Ala30-Gln289    
  Accession # NP_579929

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Prostasin/Prss8 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prostasin/Prss8

Prostasin encoded by the Prss8 gene, also known as channel activating protease 1, is a serine protease with a trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of prostasin is involved in the regulation of epithelial sodium channels (4, 5). The enzymatic activity of Prostasin is effectively inhibited by HAI-1 and HAI-2B (Catalog #  and ).

• References:

1. Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.

2. Yu J.X. et al. (1994) J. Biol. Chem. 269:18843.

3. Yu J.X. et al. (1995) J. Biol. Chem. 270:13483.

4. Caldwell R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C190.

5. Tong Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.

• Entrez Gene IDs:

  5652 (Human); 76560 (Mouse)

• Alternate Names:

  CAP1; channel-activating protease 1; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.120; Prostasin; protease, serine, 8; Prss8; Serine protease 8