• Species Reactivity

  Mouse

• Specificity

  Detects mouse CD5.

• Source

  Monoclonal Rat IgG2a Clone # 53-7.3

• Immunogen

  Mouse thymus or spleen

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 594

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 µg/10    ⁶ cells

  Mouse splenocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD5

Mouse CD5 has been shown to react with a mouse monomorphic CD5 (Ly-1), a Group B member of the scavenger receptor cysteine-rich protein superfamily (1). CD5 is a 67 kDa type I transmembrane glycoprotein found on thymocytes, T cells, and a subset of B cells but not on NK cells (2‑6). It is likely a ligand of the B cell differentiation antigen CD72 and is known to associate with CD6 (1). Mouse and human extracellular domains share 55% amino acid sequence indentity.

• References:

1. Martinez, V.G., et al. (2011) Pharmacol. Rev. 63:967.

2. Ledbetter, J.A. et al. (1980) J. Exp. Med. 152:280.

3. van Ewijk, W. et al. (1981) J. Immunol. 127:2594.

4. Hayakawa, K. et al. (1983) J. Exp. Med. 157:202.

5. Luo, W. et al. (1992) J. Immunol. 148:1630.

6. Lanier, L.L. et al. (1986) J. Immunol. 137:2735.

• Entrez Gene IDs:

  921 (Human); 12507 (Mouse)

• Alternate Names:

  CD5 antigen (p56-62); CD5 antigen; CD5 molecule; CD5; LEU1T-cell surface glycoprotein CD5; Lymphocyte antigen T1/Leu-1; T1