• Specificity

  Selected for use as rat IgG    _2A isotype control for flow cytometry applications.

• Source

  Monoclonal Rat IgG    _2A Clone # 54447

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  KLH

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Fluorescein

Applications

• Recommended    
  Concentration

  Sample

• Control

  Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/10    ⁶ cells.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

• Long Name:

  Immunoglobulin G2A Fc

• Alternate Names:

  IgG2A