详细说明
Species Reactivity
Human
Specificity
Detects human APJ in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG 1 Clone # 72133R-G1
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human APJ
Met1-Asp380
Accession # P35414Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin
Applications
Recommended
ConcentrationSample
Flow Cytometry
10 µL/10 6 cells
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Flow Cytometry | Detection of APJ in U‑118‑MG Human Cell Line by Flow Cytometry. U‑118‑MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human APJ APC‑conjugated Monoclonal Antibody (Catalog # FAB8561A, filled histogram) or isotype control antibody (Catalog # , open histogram). View our protocol for . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: APJ
APJ is a G-protein coupled receptor that is related to the angiotensin receptor. It is primarily expressed in coronary artery endothelium and binds the endogenous ligand, apelin. APJ can also function as a co-receptor for HIV.
Long Name:
Apelin Receptor/G-protein Coupled Receptor APJ
Entrez Gene IDs:
187 (Human); 23796 (Mouse)
Alternate Names:
AGTRL1; AGTRL1HG11; angiotensin II receptor-like 1; Angiotensin receptor-like 1; apelin receptor; APJ (apelin) receptor; APJ receptor; APJ; APJFLJ96609; APJR; APLNR; FLJ90771; G protein-coupled receptor APJ; G-protein coupled receptor APJ; G-protein coupled receptor HG11; HG11 orphan receptor; HG11; MGC45246