• Species Reactivity

  Mouse

• Specificity

  Detects mouse Lgr5/GPR49 in direct ELISAs. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in Flow Cytometry.

• Source

  Monoclonal Rat IgG    _2B Clone # 889901

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse Lgr5/GPR49 N-terminal peptide    
  Ala21-Cys34    
  Accession # Q9Z1P4

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Phycoerythrin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.1 µg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Lgr5/GPR49

Leucine‑rich repeat G‑protein‑coupled Receptor 5 (Lgr5), also known as GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling. Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells. Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts. It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas.

• Long Name:

  Leucine-rich Repeat Containing G Protein-coupled Receptor 5

• Entrez Gene IDs:

  8549 (Human); 14160 (Mouse); 299802 (Rat)

• Alternate Names:

  FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38