• Species Reactivity

  Human

• Specificity

  Detects human Aminopeptidase LRAP/ERAP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human PILS is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 3F5

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human Aminopeptidase LRAP/ERAP2    
  Accession # Q6P179

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human Aminopeptidase LRAP/ERAP2 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminopeptidase LRAP/ERAP2

Leukocyte-derived arginine aminopeptidase (LRAP) and endoplasmic reticulum-aminopeptidase 2 (ERAP2) are the two names given to the protein encoded by the LRAP gene (1, 2). Induced by interferon-gamma, LRAP is able to trim various MHC class I antigenic peptide precursors. It belongs to the oxytocinase subfamily of M1 aminopeptidases, which also includes aminopeptidases PILS/ARTS1/ERAP1 and LNPEP/PLAP (1, 3). In addition to antigen presentation, the members of this subfamily are also important in maintenance of pregnancy, memory retention, and blood pressure regulation (3).

• References:

1. Tanioka, T. et al. (2003) J. Biol. Chem. 278:32275.

2. Tanioka, T. et al. (2005) FEBS J. 272:916.

3. Tsujimoto, M. and A. Hattori (2005) Biochim. Biophys. Acta 1751:9.

• Long Name:

  Leukocyte-derived Arginine Aminopeptidase

• Entrez Gene IDs:

  64167 (Human)

• Alternate Names:

  Aminopeptidase LRAP; EC 3.4.11; EC 3.4.11.3; endoplasmic reticulum aminopeptidase 2; ERAP2; FLJ23633; FLJ23701; FLJ23807; Leukocyte-derived arginine aminopeptidase; LRAPL-RAPEC 3.4.11.-