• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse R‑Spondin 3 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) R‑Spondin 1, recombinant human R‑Spondin 2, or rmR‑Spondin 4 is observed.

• Source

  Monoclonal Rat IgG    _2B Clone # 400403

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse R-Spondin 3    
  Ser21-Gly209 (Gln108Lys)    
  Accession # BAC36296

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human R-Spondin 3 (Catalog # )

  Recombinant Mouse R-Spondin 3 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: R-Spondin 3

R-Spondin 3 (RSPO3, roof plate-specific spondin 3), also called cysteine-rich and single thrombospondin domain containing-1 (Cristin 1), is an ~31 kDa secreted protein that shares ~40% amino acid (aa) identity with the other three R-Spondin family members (1, 2). All are positive modulators of Wnt/ beta -catenin signaling, but each has a distinct expression pattern (1‑4). Like other R-spondins, R-Spondin 3 contains two adjacent cysteine-rich furin-like domains (aa 35‑135) with one potential N-glycosylation site (aa 36), followed by a thrombospondin (TSP-1) motif (aa 147‑207) and a region rich in basic residues (aa 211‑269). Only the furin-like domains are needed for beta -catenin stabilization (2). Within aa 21‑209, human R-Spondin 3 shares 93%, 92%, 97%, 96% and 92% aa identity with mouse, rat, equine, bovine and canine R-Spondin 3, respectively. Potential isoforms of 279 and 297 aa diverge at aa 210 and 276, respectively (5). Mouse R-Spondin 3 is critical for development of the placental labyrinthine layer, probably by promoting VEGF expression and thus vascular development (6, 7). It is also essential for expression of the placenta‑specific transcription factor, Gcm1. In the mouse embryo, R-Spondin 3 is often expressed by or located near endothelial cells (6). It is found in the roof plate, tail, somites, otic vesicles, cephalic mesoderm, truncus arteriosus, atrioventricular canal of the developing heart, and strongly but transiently in developing limbs (4, 7). R‑Spondins regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptors LRP-6 and Kremen, reducing their DKK-1-mediated internalization (8, 9). Reports differ on whether R-Spondins bind LRP-6 directly (8‑10). R-Spondin 3 has also been identified as an oncogene (11).

• References:

1. Chen, J-Z. et al. (2002) Mol. Biol. Rep. 29:287.

2. Kim, K.-A. et al. (2008) Mol. Biol. Cell 19:2588.

3. Hendrickx, M. and L. Leyns (2008) Develop. Growth Differ. 50:229.

4. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.

5. Entrez Accession # EAW48114 and EAW48116.

6. Kazanskaya, O. et al. (2008) Development 135:3655.

7. Aoki, M. et al. (2007) Dev. Biol. 301:218.

8. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.

9. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.

10. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.

11. Theodorou, V. et al. (2007) Nat. Genet. 6:759.

• Long Name:

  Roof Plate-specific Spondin 3

• Entrez Gene IDs:

  84870 (Human); 72780 (Mouse)

• Alternate Names:

  Cristin 1; CRISTIN1; FLJ14440; hPWTSR; hRspo3; Protein with TSP type-1 repeat; PWTSR; Roof plate-specific spondin-3; RSPO3; R-spondin 3 homolog (Xenopus laevis); RSpondin 3; R-Spondin 3; R-spondin-3; Thrombospondin type-1 domain-containing protein 2; thrombospondin, type I, domain containing 2; THSD2