• Species Reactivity

  Human

• Specificity

  Detects human ATG4B/Apg4b in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human ATG4A is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 515803

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human ATG4B/Apg4b    
  Asp2-Leu393 (predicted)    
  Accession # Q9Y4P1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human ATG4B

• 
  

• Immunoprecipitation

  25 µg/mL

  Cell lysates spiked with Recombinant Human ATG4B,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ATG4B

Autophagy related protein 4 homolog B (ATG4B), also known as autophagin 1 and Apg4b, is a cytosolic cysteine protease that is involved in autophagy, a biological process in which endogenous protein and damaged organelles are destroyed. Human ATG4B shares 98% and 95% amino acid sequence identity with chimpanzee and mouse ATG4B, respectively.

• Long Name:

  ATG4 Autophagy Related 4 Homolog B

• Entrez Gene IDs:

  23192 (Human); 66615 (Mouse); 316640 (Rat)

• Alternate Names:

  APG4 autophagy 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B; Apg4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUTL1; AUTL1MGC1353; AUT-like 1 cysteine endopeptidase; autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; cysteine protease ATG4B; DKFZp586D1822; EC 3.4.22; EC 3.4.22.-; hAPG4B; KIAA0943