• Species Reactivity

  Human, Mouse

• Specificity

  Detects human or mouse SOCS-3 in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 516919

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human SOCS-3    
  Met1-Leu225    
  Accession # O14543

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 µg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOCS-3

Suppressor of cytokine signaling 3 (SOCS-3), also known as CIS3, is a widely expressed 30 kDa protein that inhibits signal transduction induced by multiple cytokines including Erythropoietin, IL-6, LIF, Oncostatin M, and Leptin. It binds downstream signaling molecules such as JAK kinases and targets them for ubiquitin-mediated degradation, essential in a negative feedback control loop. Human SOCS-3 shares 96% aa sequence identity with mouse and rat SOCS-3.

• Long Name:

  Suppressor Of Cytokine Signaling 3

• Entrez Gene IDs:

  9021 (Human); 12702 (Mouse); 89829 (Rat)

• Alternate Names:

  ATOD4; CIS3; CIS3CIS-3; Cish3; cytokine-induced SH2 protein 3; Cytokine-inducible SH2 protein 3; SOCS3; SOCS-3; SOCS-3STAT-induced STAT inhibitor 3; SSI-3; SSI-3ATOD4; SSI3MGC71791; suppressor of cytokine signaling 3