• Species Reactivity

  Human

• Specificity

  Detects human FCRL5/FcRH5 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) FCRL1, rhFCRL2, rhFCRL3, or rhFCRL4 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 307314

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human FCRL5/FcRH5    
  Gln16-Arg844 (predicted)    
  Accession # AAI01067

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human FCRL5/FcRH5 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL5/FcRH5

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one immunotyrosine activation motif (ITAM) and two immunotyrosine inhibitory motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5‑7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the up‑regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is up‑regulated on tumor cells in some types of B cell malignancies (6, 10‑12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

• References:

1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.

2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.

3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.

4. SwissProt # Q96RD9.

5. Miller, I. et al. (2002) Blood 99:2662.

6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.

7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.

8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.

9. Mohan, J. et al. (2006) Blood 107:4433.

10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.

11. Ise, T. et al. (2007) Leukemia 21:169.

12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.

13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.

• Long Name:

  Fc Receptor-like 5

• Entrez Gene IDs:

  83416 (Human); 329693 (Mouse)

• Alternate Names:

  BXMAS1; BXMAS1MGC119592; CD307; CD307e antigen; CD307e; DKFZp667E2019; DKFZp667F216; Fc receptor homolog 5; Fc receptor-like 5; Fc receptor-like protein 5; FcRH5; FCRH5MGC119593; FCRL5; FcR-like protein 5; FLJ00333; FLJ00397; IFGP5; Immune receptor translocation-associated protein 2; immunoglobulin superfamily receptor translocation associated 2 (IRTA2); IRTA2; IRTA2MGC119590; PRO820