• Species Reactivity

  Human, Mouse

• Specificity

  Detects human Carbonic Anhydrase VIII/CA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CA1, 2, 3, 4, 9, 10, 12, 13, or 14 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 308320

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Carbonic Anhydrase VIII/CA8    
  Ala2-Gln290    
  Accession # P35219

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase VIII/CA8

Carbonic Anhydrase VIII (CA8), also called CA‑related protein (CARP), is a cytosolic protein without CA activity (i.e., the reversible hydration of CO₂) due to point mutations in the zinc-binding site (1, 2). Nevertheless, it has esterase activity. CA8 is expressed exclusively in Purkinje cells of the cerebellum, where it binds inositol 1,4,5-triphosphate receptor type 1 (3). CA8 overexpression in human colorectal cancer and non-small cell lung cancer indicates that it plays a role in the process of invasion in these types of malignancy (4, 5). The 289 amino acid rhCA8 has a predicted molecular mass of approximately 33 kDa. It shares 98% amino acid identity with mouse, rat and bovine CA8 and 78% with Xenopus.

• References:

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.

2. Sjoblom, J. et al. (1996) FEBS Lett. 398: 322.

3. Hirota, J. et al. (2003) Biochem. J. 372: 435.

4. Miyaji, E. et al. (2003) J. Pathol. 201: 37.

5. Lu, S.H. et al. (2004) Lung Cancer 44: 273.

• Entrez Gene IDs:

  767 (Human)

• Alternate Names:

  CA8; CALS; CALScarbonate dehydratase; CAMRQ3; Carbonic Anhydrase VIII; carbonic anhydrase VIIIMGC99509; carbonic anhydrase-like sequence; carbonic anhydrase-related protein; CARP; CARPCA-related protein; CA-VIII; MGC120502