Human FABP1/L-FABP MAb (Clone 328607) 100 UG

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Human FABP1/L-FABP MAb (Clone 328607) 100 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human, Mouse, Rat

    • Specificity

      Detects human FABP1/L‑FABP in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human FABP2, -3, -4, -5, -6, -7, or recombinant mouse FABP9 is observed.

    • Source

      Monoclonal Mouse IgG    2A Clone # 328607

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      E. coli-derived recombinant human FABP1/L‑FABP    
      Met1-Ile127    
      Accession # AAA52418

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.25 µg/mL

      See below


    • Simple Western

      5 µg/mL

      See below


    • Immunohistochemistry

      8-25 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human FABP1/L‑FABP by Western Blot. Western blot shows lysates of human liver tissue, human colon tissue, and human kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB2964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ).
    A specific band was detected for FABP1/
    L‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Western Blot      
         

    Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/
    L‑FABP Monoclonal Antibody (Catalog # MAB2964) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # ). A specific band was detected for FABP1/L‑FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Immunohistochemistry      
         

    FABP1/L‑FABP in Human Kidney. FABP1/L‑FABP was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human/Mouse/Rat FABP1/L‑FABP Monoclonal Antibody (Catalog # MAB2964) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # ) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for .

    Simple Western      
         

    Detection of Mouse FABP1/L‑FABP by Simple WesternTM. Simple Western lane view shows lysates of mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP1/L‑FABP at approximately 16 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse/Rat FABP1/
    L‑FABP Monoclonal Antibody (Catalog # MAB2964). This experiment was conducted under reducing conditions and using the
    12-230 kDa separation system.

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: FABP1/L-FABP

    FABP1, also known as liver FABP (L-FABP, Z-protein and squalene-and sterol-carrier protein [SCP]) is a member of the intracellular FABP family. It is highly expressed in the liver, intestine, kidney and lung. FABP1 binds free fatty acids and their co-enzyme A derivatives and may be involved in intracellular lipid transport.

    • Long Name:

      Fatty Acid-Binding Protein 1

    • Entrez Gene IDs:

      2168 (Human); 14080 (Mouse); 24360 (Rat)

    • Alternate Names:

      FABP1; FABPL; fatty acid binding protein 1, liver; fatty acid-binding protein, liver; LFABP; L-FABP; L-FABPFatty acid-binding protein 1; Liver-type fatty acid-binding protein









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