• Species Reactivity

  Mouse

• Specificity

  Detects mouse Chordin-like 2/CHRDL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CHRDL2, recombinant mouse (rm) CHRDL1, or rmChordin is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 328905

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Chordin-like 2/CHRDL2    
  Gln24-Leu426    
  Accession # Q8VEA6.1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse Chordin-Like 2/CHRDL2 (Catalog # )

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Mouse Chordin-like 2/CHRDL2 (Catalog # ),

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chordin-like 2/CHRDL2

Chordin-like 2 (CHL2, CHRDL2, or BNF-1), is a secreted glycoprotein that functions as a BMP antagonist. It is expressed in chondrocytes in developing joint cartilage, osteoblasts, myotubes, brain, some carcinomas, and the genitourinary system (e.g. uterus, prostate, testis, bladder). CHL2 binds to multiple TGF-beta superfamily proteins including BMP-2, -4, -5, -6, -7, GDF-5, Activin A, and TSG. It prevents the binding of BMPs to type 1 and type 2 receptors as well as BMP-induced cellular responses. CHL2 reduces the rate of matrix deposition by mesenchymal cells, acting as a negative regulator of cartilage formation.

• Entrez Gene IDs:

  25884 (Human); 69121 (Mouse)

• Alternate Names:

  BNF-1; Chordin like 2; Chordin-like 2; chordin-like protein 2; CHRDL2