• Species Reactivity

  Human, Mouse

• Specificity

  Detects recombinant human or mouse Sulfatase Modifying Factor 1/SUMF1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SUMF2 or recombinant mouse SUMF2 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 329005

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Sulfatase Modifying Factor 1/SUMF1 isoform 1    
  Ser34-Asp374 (Ser63Asn)    
  Accession # Q8NBK3.3

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human and mouse Sulfatase Modifying Factor 1/SUMF1

• 
  

• Immunoprecipitation

  25 µg/mL

  Conditioned cell culture medium spiked with Recombinant Human or Recombinant Mouse Sulfatase Modifying Factor 1/SUMF1,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sulfatase Modifying Factor 1/SUMF1

Also known as C-alpha -formylglycine-generating enzyme (FGE), SUMF1 converts the active site Cys residue of sulfatases into formylglycine, which is essential for sulfatase activity. SUMF1 defect causes multiple sulfatase deficiency (MSD). The amino acid sequence of rhSUMF1 is 93%, 92% and 90% identical to that of canine, bovine, and mouse/rat.

• Entrez Gene IDs:

  285362 (Human); 58911 (Mouse); 362409 (Rat)

• Alternate Names:

  AAPA3037; EC 1.8.99.-; FGE1; FGEC-alpha-formylglycine-generating enzyme 1; FGly-generating enzyme; MGC131853; MGC150436; sulfatase modifying factor 1; sulfatase-modifying factor 1; SUMF1; UNQ3037