• Species Reactivity

  Human

• Specificity

  Detects human DSPG3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse DSPG3 is observed.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 329707

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human DSPG3    
  Ala20-Val322    
  Accession # Q99645

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human DSPG3 (Catalog # )

• 
  

• Immunocytochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSPG3

Human DSPG3 (also PG-Lb and epiphycan) is a 95‑100 kDa class III subfamily member of the SLRP family of molecules. It is synthesized as a 322 aa precursor that contains a 19 aa signal sequence and a 303 aa mature region. The mature molecule contains two intrachain disulfide bonds that flank six leucine-rich repeats lying between aa 135-301. There is a potential for both O- and N-linked glycosylation. DSPG3 is found principally in cartilage. Mature human DSPG3 is 85%, 96%, 95% and 84% aa identical to rat, dog, cow and mouse DSPG3, respectively.

• Long Name:

  Dermatan Sulfate Proteoglycan 3

• Entrez Gene IDs:

  1833 (Human); 13516 (Mouse)

• Alternate Names:

  Dermatan sulfate proteoglycan 3dermatan sulphate proteoglycan 3; DSPG3; DSPG3proteoglycan-lb; Epiphycan; EPYC; PGLB; PG-Lb; Proteoglycan-Lb; SLRR3B; SLRR3Bepiphycan proteoglycan; Small chondroitin/dermatan sulfate proteoglycan