• Species Reactivity

  Human

• Specificity

  Detects human IL-17 RB in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL‑17 RB, recombinant human IL-17 R, or rmIL-17 R.

• Source

  Monoclonal Mouse IgG    _2B Clone # 170220

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IL-17 RB    
  Arg18-Gly289    
  Accession # Q9NRM6

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Allophycocyanin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 µL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17 RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1). Interleukin-17 B Receptor (IL-17 RB), also known as IL-17Rh1, IL-17ER and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17 RB has also been identified (4). Human and mouse IIL-17 RB share 76% aa sequence identity. The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17 RB is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17 RB is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2-4).

• References:

1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.

2. Shi Y, et al. (2000) J. Biol. Chem. 275:19167.

3. Lee, J, et al. (2001) J. Biol. Chem. 276:1660.

4. Tian E, et al. (2000) Oncogene 19:2098.

5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.

6. Hurst, S.D. et al. (2002) J. Immunol. 169:443.

• Long Name:

  Interleukin 17B Receptor

• Entrez Gene IDs:

  55540 (Human); 50905 (Mouse)

• Alternate Names:

  CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor