• Species Reactivity

  Mouse

• Specificity

  Detects mouse CLEC4F/CLECSF13 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) CLECSF4F, rhCLEC4D, rhCLEC4E, recombinant mouse (rm) OCIL, and rmOCIL-rp2 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 370901

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse CLEC4F/CLECSF13    
  Ala65-Gly548    
  Accession # P70194

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Mouse CLEC4F/CLECSF13 (Catalog # )

• 
  

• Immunohistochemistry

  8-25 µg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC4F/CLECSF13

CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily (1‑3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N‑acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).

• References:

1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.

2. Bilzer, M. et al. (2006) Liver Int. 26:1175.

3. Kuiper, J. et al. (1994) Biochem. J. 299:285.

4. Accession # P70194.

5. Hoyle, G.W. and R.L. Hill (1988) J. Biol. Chem. 263:7487.

6. Fadden, A.J. et al. (2003) Glycobiology 13:529.

7. Beavil, A.J. et al. (1992) Proc. Natl. Acad. Sci. 89:753.

8. Haltiwanger, R.S. et al. (1986) J. Biol. Chem. 261:7433.

9. Coombs, P.J. et al. (2006) Glycobiology 16:1C.

10. Biessen, E.A.L. et al. (1994) Biochem. J. 299:291.

11. Lehrman, M.A. et al. (1986) J. Biol. Chem. 261:7426.

• Long Name:

  C-type Lectin Domain Family 4, Member F

• Entrez Gene IDs:

  165530 (Human); 51811 (Mouse); 114598 (Rat)

• Alternate Names:

  CLEC4F; CLECSF13; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13; C-type lectin 13; C-type lectin domain family 4, member F; Galactose Particle Receptor; KCLR; KCR