• Species Reactivity

  Human

• Specificity

  Detects human TEM8/ANTXR1 in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 371113

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TEM8/ANTXR1 isoform 1    
  Gln28-Gly320    
  Accession # Q9H6X2

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 µg/mL

  Recombinant Human TEM8/ANTXR1

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TEM8/ANTXR1

Tumor Endothelial Marker 8 (TEM8), also called ATR1 and ANTXR1, is a glycoprotein of the anthrax toxin receptor family that is expressed by endothelial cells. Type I transmembrane isoforms of 564 aa (80‑85 kDa) and 368 aa (60 kDa) and potentially secreted isoforms of 330 aa and 297 aa (45 kDa) are differentially expressed. All diverge at the C-terminal end but share the N-terminal vWFA domain that binds the anthrax toxin PA subunit as well as collagens I and IV. Human TEM8 shares 99% aa identity with mouse and rat and 92% identity with dog and chick TEM8 within the extracellular domain.

• Long Name:

  Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1

• Entrez Gene IDs:

  84168 (Human); 69538 (Mouse); 362393 (Rat)

• Alternate Names:

  anthrax toxin receptor 1; ANTXR1; ATR2310008J16Rik; FLJ10601; FLJ11298; FLJ21776; TEM8; TEM82810405N18Rik; Tumor endothelial marker 8