Mouse AMIGO3 MAb (Clone 371301) 100 UG

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Mouse AMIGO3 MAb (Clone 371301) 100 UG信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Mouse

    • Specificity

      Detects mouse AMIGO3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse AMIGO2, recombinant human (rh) AMIGO1, or rhAMIGO2 is observed.

    • Source

      Monoclonal Rat IgG    2A Clone # 371301

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Mouse myeloma cell line NS0-derived recombinant mouse AMIGO3    
      Thr20-Pro378    
      Accession # Q8C2S7

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 µg/mL

      Recombinant Mouse AMIGO3


    • Immunohistochemistry

      8-25 µg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Immunohistochemistry      
         

    AMIGO3 in Mouse Brain. AMIGO3 was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using 25 µg/mL Rat Anti-Mouse AMIGO3 Monoclonal Antibody (Catalog # MAB2375) overnight at 4 °C. Tissue was stained (red). View our protocol for .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: AMIGO3

    AMIGO3 (Amphoterin-Induced Gene and ORF 3) is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine-rich repeats (CRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied. AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and 2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with that of rat and human AMIGO3, respectively.

    • Long Name:

      Amphoterin-induced Gene and ORF 3

    • Entrez Gene IDs:

      386724 (Human); 320844 (Mouse)

    • Alternate Names:

      adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552

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