详细说明
Species Reactivity
Human
Specificity
Detects human IL-9 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1043G
Purification
Protein A or G purified from cell culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-9
Gln19-Ile144
Accession # P15248Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Applications
Recommended
ConcentrationSample
Western Blot
1:1000 dilution
See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
Western Blot | Detection of Human IL‑9 by Western Blot. Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # ), Recombinant Human IL‑4 (Catalog # ), and Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # ) or (3) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # ), Recombinant Human IL‑4 (Catalog # ), Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # ), and Recombinant Human TGF‑ beta 1 (Catalog # ). PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human IL‑9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for IL‑9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using . |
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
12 months from date of receipt, -20 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 °C under sterile conditions after opening.
Background: IL-9
Interleukin-9 (IL-9) is a heavily glycosylated cytokine with a 14 kDa peptide backbone that is secreted by CD4 + Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.
Long Name:
Interleukin 9
Entrez Gene IDs:
3578 (Human); 16198 (Mouse)
Alternate Names:
Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40