Human IL-9 MAb (Clone 1043G) 100 UL

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Human IL-9 MAb (Clone 1043G) 100 UL信息二维码

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产品介绍

    基本参数

    详细说明

    • Species Reactivity

      Human

    • Specificity

      Detects human IL-9 in Western blots.

    • Source

      Recombinant Monoclonal Rabbit IgG Clone # 1043G

    • Purification

      Protein A or G purified from cell culture supernatant

    • Immunogen

      S. frugiperda insect ovarian cell line     Sf 21-derived recombinant human IL-9    
      Gln19-Ile144    
      Accession # P15248

    • Formulation

      Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1:1000 dilution

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human IL‑9 by Western Blot. Western blot shows lysates of human Th2 cells treated with (1) anti-CD3 and anti-CD28, (2) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # ), Recombinant Human IL‑4 (Catalog # ), and Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # ) or (3) anti-CD3, anti-CD28, Recombinant Human IL‑2 (Catalog # ), Recombinant Human IL‑4 (Catalog # ), Mouse Anti-Human IFN‑ gamma Monoclonal Antibody (Catalog # ), and Recombinant Human TGF‑ beta 1 (Catalog # ). PVDF membrane was probed with a 1:1000 dilution of Rabbit Anti-Human IL‑9 Monoclonal Antibody (Catalog # MAB2091) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # ). A specific band was detected for IL‑9 at approximately 35-40 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      • 12 months from date of receipt, -20 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after opening.

      • 6 months, -20 °C under sterile conditions after opening.

    Background: IL-9

    Interleukin-9 (IL-9) is a heavily glycosylated cytokine with a 14 kDa peptide backbone that is secreted by CD4  + Th2 cells. It supports the growth of multiple hematopoietic cell types including Th cells, germinal center B cells, macrophages, mast cells, neutrophils, megakaryocytes, and erythrocytes. IL-9 exerts its biological effects through a receptor complex composed of IL-9 R and the common gamma chain. Mature human IL-9 shares 57% amino acid sequence identity with mouse and rat IL-9.

    • Long Name:

      Interleukin 9

    • Entrez Gene IDs:

      3578 (Human); 16198 (Mouse)

    • Alternate Names:

      Cytokine P40; HP40; IL9; IL-9; IL-9homolog of mouse T cell and mast cell growth factor 40; interleukin 9; interleukin-9; p40 cytokine; p40 T-cell and mast cell growth factor; P40; T-cell growth factor P40






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