详细说明
- Purity>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
- ActivityRecombinant Human USP28 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human USP28 concentration of 10-500 nM.
- SourceSpodoptera frugiperda, Sf 21 (baculovirus)-derived Contains a C-terminal 6-His tag
- Accession #
- Predicted Molecular Mass123 kDa
| E-570 | | |
| Formulation X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP | ||
| Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. | ||
| Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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USP28 is a deubiquitinating enzyme of the C19 peptidase family that regulates MYC stability and DNA damage response checkpoints. USP28 regulates DNA repair by deubiquitinating and thereby stabilizing CLSPN - a protein that is ubiquitinated by the APC/C E3 Ubiquitin ligase in a cell-cycle dependent manner. USP28 interacts with isoform 1 of FBXW7 in the nucleoplasm, and regulates MYC degradation by counteracting its ubiquitination. Recently, a potential role in HIF-1 alpha -dependent processes such as angiogenesis and metastasis has been described for this deubiquitinase. USP28 has been demonstrated to cleave K6, K11, K48, and K63-linked poly-Ubiquitin chains. This recombinant protein contains a C-terminal 6-His tag.
- References:
- Basserman F., et al. (2008) Cell 134: 256-267.
- Flügel D., et al. (2012) Blood 119:1292-301.
- Popov N., et al. (2007) Cell Cycle 6: 2327-2331.
- Zhang D., et al. (2006) Cell 126: 529-542.
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- Long Name:Ubiquitin Specific Protease 28
- Entrez Gene IDs:57646 (Human); 235323 (Mouse); 315639 (Rat)
- Alternate Names:Deubiquitinating enzyme 28; EC 3.1.2.15; EC 3.4.19.12; KIAA1515ubiquitin carboxyl-terminal hydrolase 28; ubiquitin specific peptidase 28; ubiquitin specific protease 28; ubiquitin thioesterase 28; Ubiquitin thiolesterase 28; Ubiquitin-specific-processing protease 28; USP28
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