• Purity

  >95%, by SDS-PAGE with silver staining

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T.     et al. (1989) J. Cell Physiol.     140:323. The ED    ₅₀ for this effect is 0.05-0.3 ng/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Ala26-Arg221

• Accession #

• N-terminal Sequence    
  Analysis

  Ala26 & Ile28

• Predicted Molecular Mass

  22 kDa

• SDS-PAGE

  27-35 kDa, reducing conditions

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Background: Oncostatin M/OSM

Oncostatin M (OSM) is an approximately 30 KDa secreted cytokine belonging to the Interleukin-6 family. Like other members of the IL-6 family such as IL-11, CNTF, and Cardiotrophin-1, OSM plays crucial roles in inflammation, neuroprotection, hematopoiesis, metabolism and development (1). The human OSM cDNA encodes a 252 amino acid (aa) prepro protein with a signal peptide and a hydrophilic Cterminal domain that are proteolytically processed to generate the 195 aa mature form (2). Although both mature and pro-OSM are equally active in radioreceptor assays, the mature OSM is 5 to 60 fold more active in growth inhibition assays (3). Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the   in vivo activities of OSM (3). Cytokines of the IL-6 family share a common receptor subunit, gp130, which allows intracellular signal transduction. In contrast to other cytokines, OSM binds gp130 with very low affinity and has little to no biological activity unless a second receptor chain is recruited (4). The OSM-gp130 complex recruits either LIF R alpha, or OSM R beta to form the OSM receptor type I or OSM receptor type II respectively (5). Among multiples roles, OSM has been reported to modulate tumor cell and epithelial cell growth (6, 7). An axis involving OSM, CD40 Ligand and OSM receptor described as an antiviral and immunostimulatory system is disrupted in patients liver showing hepatitis C infection (8). Human OSM shares 45% aa sequence homology with mouse and rat OSM.

• References:

1. Richards, C.D. (2013) ISRN Inflamm. 2013:512103.

2. Malik, N. et al. (1989) Mol. Cel. Biol. 9:2847.

3. Linsley, P.S. et al. (1990) Mol. Cel. Biol. 10:1882.

4. Liu, J. et al. (1994) Cytokine 6:272.

5. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.

6. Grant, S.L. et al. (2001) Growth Factors 19:153.

7. Chollangi, S. et al. (2012) J. Biol. Chem. 287:32848.

8. Larrea, E. et al. (2014) J. Hepatol. 60:482.

• Entrez Gene IDs:

  5008 (Human); 18413 (Mouse)

• Alternate Names:

  MGC20461; oncostatin M; oncostatin-M; OSM