• Assay Type

  Magnetic bead-based multiplex assay for the Luminex    ^® MAGPIX    ^® platform

• Format

  96-well microplate and antibody-coated magnetic beads*

• Analytes Detected

  Please see table below.

• Performance Validation

  For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below).

* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).

Product Features

• Simultaneously detect multiple analytes

• Magnetic format allows for easier wash steps

• User selects specific analytes of interest

• Requires small plate size

• Undergoes similiar validation testing as Quantikine^® ELISA Kits

• Precise and Specific

Base Kit Contents

• Standard Cocktail(s)

• Bead Diluent

• Biotin Antibody Diluent

• Calibrator Diluent(s)

• Streptavidin-PE

• Wash Buffer Concentrate

• Mixing Bottles

• Flat-bottom Microplate

• Foil Plate Sealers

Bead Set Contents

• Antibody-coated magnetic beads

• Biotinylated detection antibody

Information for Compatiable Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Beads Sets

NOTE: Due to a low level of cross-reactivity of the biotinylated anti-TGF-β1 antibody with TGF-β2, multiplexing TGF-β1 and TGF-β2 can result in falsely elevated values for TGF-β2 in some sample types. We found in human serum samples, falsely elevated values for TGF-β2 when multiplexing TGF-β1 and TGF-β2. Similar cross-reactivity was observed in competitor’s multiplex assays. For studies using human serum samples, TGF-β1 and TGF-β3, or TGF-β2 and TGF-β3 can be multiplexed; however, careful consideration should be given when deciding to evaluate TGF-β1 and TGF-β2 as a multiplex. Testing with samples from healthy donors found the impact of the cross-reactivity observed became insignificant with human milk, platelet-poor plasma, cell culture supernates and urine samples. It is suggested that each lab evaluate their samples to determine if multiplexing will be problematic (as TGF-β levels will vary with disease state and serum source for media).

Validated Samples and Recommended Starting Dilution

More Information about Magnetic Bead-Based Multiple Kits for the Luminex Platform

Magnetic  multiplex kits are designed for use with the  CCD Imager. Alternatively, kits can be used with the  or Bio-Rad^® Bio-Plex^®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Assays for the Luminex platform are offered as  or . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit.

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