• Purity

  >95%, by SDS-PAGE with silver staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED    ₅₀ for this effect is 0.7-3.5 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human CD2F-10/SLAMF9 (Arg19-Ser210) Accession # Q96A28	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Arg19 & Val33

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  48 kDa (monomer)

• SDS-PAGE

  60-70 kDa, reducing conditions

Data Images

Background: CD2F-10/SLAMF9

The CD2 family of receptors is a subset of the immunoglobulin superfamily of glycoproteins. The CD2F-10 protein, also known as SF2001 and CD85-H1, was identified as a CD2 subfamily member using genome sequence data. Based on its structural organization, CD2F-10 belongs to the SLAM (Signaling Lymphocyte Activation Molecule) subfamily of CD2 receptors and is designated SLAMF9. SLAM proteins function as co-receptors for lymphocyte activation and/or adhesion (1). Mature human CD2F-10 is a type I transmembrane glycoprotein that consists of a 218 amino acid (aa) extracellular domain (ECD) with one Ig-like C2-set domain, a 21 aa transmembrane segment, and a 32 aa cytoplasmic domain (2-4). Unlike other SLAM proteins, the cytoplasmic domain of CD2F-10 does not contain consensus immunoreceptor tyrosine-based switch motifs (ITSMs) that mediate interactions with the signal transduction proteins SAP or EAT-2 (1). Within aa 19-210, human CD2F-10 shares 57% and 59% aa sequence identity with mouse and rat CD2F-10, respectively. Alternative splicing of human CD2F-10 generates an isoform that lacks the Ig-like C2-set domain. CD2F-10 is expressed in hematopoietic tissues and cell lines (3). R&D in house data indicate that CD2F-10 inhibits the secretion of IL-2 from activated human T cells.

• References:

1. Cannons, J.L. et al. (2011) Annu. Rev. Immunol. 29:665.

2. Fennelly, J.A. et al. (2001) Immunogenetics 53:599.

3. Zhang, W. et al. (2001) Clin. Cancer Res. 7:822s.

4. Fraser, C.C. et al. (2002) Immunogenetics 53:843.

• Long Name:

  CD2 Family 10/SLAM Family Member 9

• Entrez Gene IDs:

  89886 (Human); 98365 (Mouse)

• Alternate Names:

  CD2 family member 10; CD2F10; CD2F-10; CD2F-10MGC88194; CD84 homolog 1; CD84H1; CD84-H1; CD84-H1cluster of differentiation 2 antigen family member 10; cluster of differentiation 84 homolog 1; SF2001; signaling lymphocytic activation molecule family member 2001; signaling lymphocytic activation molecule family member 9; SLAM family member 9; SLAMF9