详细说明
- SpecificityStains N-terminal,C-terminal, and Internal Sequence HA-tagged protein transfectants but not irrelevant transfectants in flow cytometry.
- SourceRecombinant Monoclonal Mouse IgG 1 Clone # 912426
- PurificationProtein A or G purified from cell culture supernatant
- ImmunogenHA tag peptide
Accession # ABB51961 - FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 647
- Recommended
ConcentrationSample
- Intracellular Staining by Flow Cytometry5 µL/10 6 cellsSee below
Intracellular Staining by Flow Cytometry | Detection of HA Tag in HEK293 Human Cell Line Transfected withHA-tagged Proteins by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) HA-tagged proteins (filled histogram) or (B) irrelevant transfectants (open histogram) was stained with Mouse Anti-HATag Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC6875R). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # ). View our protocol for . |
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
- Entrez Gene IDs:ABB51961.1 (Viral)
- Alternate Names:HA Epitope Tag; HA Tag; HA; Hemagglutinin