详细说明
- Species ReactivityHuman
- SpecificityRecognizes the epsilon -chain of the CD3/T cell antigen receptor complex (McMichael, A.J. et al. (1987) Leucocyte Typing III: White Cell Differentiation Antigens, Oxford University Press, New York; Knapp, W. et al. (1989) Leucocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York; Schlossman, S. et al. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York).
- SourceMonoclonal Mouse IgG 1 Clone # UCHT1
- PurificationProtein A or G purified from hybridoma culture supernatant
- ImmunogenHuman thymocytes (1)
- FormulationSupplied in a saline solution containing BSA and Sodium Azide.
- LabelAlexa Fluor 647
Applications
- Recommended
ConcentrationSample
- Flow Cytometry5 µL/10 6 cellsSee below
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Data Examples
| Flow Cytometry | Detection of CD3 epsilon in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD19 PE‑conjugated Monoclonal Antibody (Catalog # ) and either (A) Mouse Anti-Human CD3 epsilon Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB100R) or (B) Mouse IgG1 Alexa Fluor 647 Isotype Control (Catalog # ). View our protocol for . |
Preparation and Storage
- ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
- Stability & StorageProtect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD3 epsilon
CD3 epsilon is one of at least four invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
- References:
- Beverly, P.C.L. and R.E. Callard (1981) Eur. J. Immunol. 11:329.
- Entrez Gene IDs:916 (Human); 12503 (Mouse)
- Alternate Names:CD3 epsilon; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; FLJ18683; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
400-6226-992