• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

• Activity

  Recombinant Human His6-UBE2N/Ubc13 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UBE2N/Ubc13 concentration of 0.1-1 μM.

• Source

  E. coli-derived Contains a 6-His tag

• Accession #

• Predicted Molecular Mass

  19 kDa

Background: UBE2N/Ubc13

Ubiquitin-conjugating Enzyme E2N (UBE2N), also known as Ubiquitin-conjugating Enzyme 13 (Ubc13), is a member of the Ubiquitin-conjugating (E2) enzyme family. UBE2N/Ubc13 has a predicted molecular weight of 17 kDa. The human enzyme shares 100% and 99% amino acid sequence identity with its mouse and rat orthologs, respectively. UBE2N/Ubc13 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (1). UBE2N/Ubc13 localizes to both the nucleus and cytoplasm (2). UBE2N/Ubc13 forms heterodimeric complexes with Uev1a/UBE2V1 and Mms2, both of which are catalytically inactive E2 enzyme variants. The UBE2N (Ubc13)/Uev1A Complex is found in the cytoplasm and is important for inflammatory responses via Nuclear Factor  kappa B (NF-kappa B) activation (3,4). In contrast, the UBE2N/Ubc13-Mms2 complex functions in the nucleus and is required for an efficient DNA damage response (3,5-7). Pathologically, UBE2N (Ubc13)/Uev1a Complex-mediated NF-kappa B activation is required for the proliferation of diffuse large B-cell lymphoma cells (8). This protein has an N-terminal His₆-tag.

• References:

   

1. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.

2. Laine, A. et al. (2006) Mol. Cell. Biol. 26:8901.

3. Andersen, P.L. et al. (2005) J. Cell Biol. 170:745.

4. Pertel, T. et al. (2011) Nature 472:361.

5. Zhao, G.Y. et al. (2007) Mol. Cell 25:663.

6. Stewart, G.S. et al. (2009) Cell 136:420.

7. Campbell, S.J. et al. (2012) J. Biol. Chem. 287:23900.

8. Pulvino, M. et al. (2012) Blood 120:1668.

• Entrez Gene IDs:

  7334 (Human); 93765 (Mouse); 116725 (Rat)

• Alternate Names:

  bendless-like ubiquitin conjugating enzyme; Bendless-like ubiquitin-conjugating enzyme; BLU; EC 6.3.2.19; MGC131857; MGC8489; UBC13; UbcH13; UbcH-ben; UBE2N; Ubiquitin carrier protein N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); Ubiquitin-protein ligase N; yeast UBC13 homolog