• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain

• Activity

  Recombinant Human His₆-­UBE2N/UBE2V2 Complex is a member of the Ubiquitin­ conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin­ activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.  We recommend an initial His_6­-UBE2N/UBE2V2 Complex concentration of 0.1­-1 μM.

• Source

  E. coli-derived Contains an N-terminal 6-His tag Accession # P61088 (UBE2N)/Q15819 (UBE2V2)

• Accession #

• Predicted Molecular Mass

  18 kDa (UBE2N), 17 kDa (UBE2V2)

Background: UBE2N/UBE2V2 Complex

Ubiquitin ­conjugating Enzyme E2N (UBE2N), also known as Ubiquitin­ conjugating Enzyme 13 (Ubc13), forms a functional complex with the catalytically inactive UBE2V2 (human homologue of yeast MMS2) protein (1). Human UBE2N/Ubc13 shares 100% and 99% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively, while human UBE2V2 shares 99% aa sequence identity with its mouse and rat orthologs. The UBE2N/UBE2V2 Complex functions with Ubiquitin ligases (E3s), including RNF111 and RNF8, to synthesize Lys63-­linked Ubiquitin chains (2,3) that can either be unanchored or attached to target proteins (4, 5). The UBE2N/UBE2V2 complex has important roles in facilitating responses to various forms of DNA damage (2, 6).

• References:

1. Hofmann R.M.& Pickart C.M. (1999) Cell          96: 645

2. Poulsen, S.L         et al. (2013) Journ Cell Biol.         201: 797

3. Campbell, S.J.         et al. (2012) Journ. Biol. Chem.         287: 23900

4. Xia, Z.P.　        et al.　(2009)　Nature　        461:114.　

5. Liu, C.         et al.　(2009)　Sci. Signal.          2:ra63.　

6. Wen, R.         et al. (2012) DNA Repair         11: 157

• Alternate Names:

  UBE2N/UBE2V2 Complex